Additional Cell Culture Reagents and Supplements
ILEX LIFE SCIENCES LLC MEM HBSS P04-10500 500 ML
PAN-Biotech MEM Eagle Cell Culture Medium w: HBSS, w: L-Glutamine, w: 0.35 g/L NaHCO3 (500 ml). Contains Hank’s Balanced Salt Solution (HBSS). BICARBONATE: With 0.35 g/L Sodium Bicarbonate. GLUCOSE: With glucose. GLUTAMINE: With L-glutamine. HEPES: Without HEPES. PHENOL RED: With Phenol Red. SODIUM PYRUVATE: Without Sodium Pyruvate. STERILE: Yes. STORAGE TEMP: +2-8°C. MANUFACTURER: PAN-Biotech GmbH. COUNTRY OF ORIGIN: Germany. DESCRIPTION: Minimum Essential Medium (MEM), developed by Harry Eagle, is a widely used synthetic cell culture media. After finding that Eagle's Basal Medium (BME) did not meet specific nutritional requirements of normal mammalian fibroblasts and certain HeLa cells, MEM was created with higher concentrations of amino acids to more closely resemble mammalian cell protein composition. When supplemented with serum, MEM is suitable for the cultivation of a wide variety of cells grown in monolayers. MEM often serves as a base medium for further modifications.
Sigma Aldrich Fine Chemicals Biosciences Hanks' Balanced Salt solution Modified, with sodium bicarbonate, without phenol red, liquid, sterile-filtered, suitable for cell culture | 6X1L
Hanks' Balanced Salt solution Modified, with sodium bicarbonate, without phenol red, liquid, sterile-filtered, suitable for cell culture | 6X1L
Sigma Aldrich Fine Chemicals Biosciences Poly-L-lysine hydrobromide mol wt 1,000-5,000 | 25988-63-0 | MFCD00237230 | 100MG
Poly-L-lysine hydrobromide mol wt 1,000-5,000 | 25988-63-0 | MFCD00237230 | 100MG
Sigma Aldrich Fine Chemicals Biosciences Holo-Transferrin Human | 11096-37-0 | MFCD00131337 | 100mg
Holo-Transferrin Human | Purity: 98%|11096-37-0 | MFCD00131337 | 100mg
Mabtech Inc Mouse Memory B-Cell Stimpack
Stimulation of B cells for use in mouse IgA, IgG and IgM ELISpot and FluoroSpot.
Sigma Aldrich Fine Chemicals Biosciences Fatty Acid Supplement Animal-component free, liquid, sterile-filtered, suitable for cell culture | 5ML
Fatty Acid Supplement Animal-component free, liquid, sterile-filtered, suitable for cell culture | 5ML
STEMCELL Technologies ImmunoCult™-ACF Dendritic Cell Differentiation Supplement, Size: 1 mL
Supplement for the differentiation of human monocytes into dendritic cells
STEMCELL Technologies NeuroCult™ SM1 Without Insulin, Size: 10 mL
NeuroCult™ SM1 Without Insulin is an optimized serum-free culture supplement, based on the published B27 formulation (Brewer et al.), with insulin removed. NeuroCult™ SM1 Without Insulin can be used in protocols where the effects of insulin are being studied, or where insulin may interfere with cellular function. In combination with a basal medium of choice, NeuroCult™ SM1 Without Insulin can be used for neural progenitor cell expansion, the study of neural stem and progenitor cell differentiation, and cardiomyocyte and hepatocyte differentiation protocols.
Sigma Aldrich Fine Chemicals Biosciences apo-Transferrin human | 11096-37-0 | MFCD00130536 | 500mg
apo-Transferrin human | Purity: 98%MW: | 11096-37-0 | MFCD00130536 | 500mg
ATHENA ES Augmedium™, Powder 100
Augmedium™ is a medium additive which conditions cells prior to induction during a recombinant protein expression protocol. This pre-induction conditioner increases the level of chaperone proteins which can improve the fraction of product that accumulates as soluble protein. Augmedium™ is specifically intended for use with strains in which the target protein accumulates as an inclusion body or as an insoluble aggregate. Augmedium™ is supplied as a powder for preparation of 50x concentrated stock solutions. It is available in 100mL and 500mL amounts.
Sigma Aldrich Fine Chemicals Biosciences Poly-L-lysine hydrobromide mol wt 70,000-150,000 by viscosity | 25988-63-0 | MFCD00237230 | 500mg
Poly-L-lysine hydrobromide mol wt 70,000-150,000 by viscosity | 627.257 | 25988-63-0 | MFCD00237230 | 500mg
Sigma Aldrich Fine Chemicals Biosciences Poly-L-lysine hydrobromide mol wt 150,000-300,000 | 25988-63-0 | MFCD00237230 | 100MG
Poly-L-lysine hydrobromide mol wt 150,000-300,000 | 25988-63-0 | MFCD00237230 | 100MG
Sigma Aldrich Fine Chemicals Biosciences Trypan Blue72-57-1 | MFCD00003969 | 5g
Trypan Blue | MW:960.81 | 72-57-1 | MFCD00003969 | 5g
Abcam 3T3-L1 Differentiation Kit, 100ML
This 3T3-L1 Differentiation Kit provides enough supplements to make 100 ml of differentiation medium and 600 ml of maintenance medium which is sufficient material for 12 100 mm culture dishes. The Differentiation Cocktail provides a final concentration of 1.5 μg/ml insulin insulin.
The product is subject to the following: Abcam Restricted Use Statement
Diagnocine LLC iPGell
Making a section of floating cells or 3D cultured cells (such as embryoid body, spheroid, etc) is burdensome work for researchers. Conventional methods are cytospin, smear slide, or agarose gel but these method gives damages to your cell sample as the cells are dried or heated during sample preparaiton. Moreover, for cytospin or smear slide method, you need to start again from sample preparation. iPGell will greatly help you to achieve this just by using two components. Cells can be rapidly jellified with unheated condition. Any toxic components affect to cells are not contained. Jellified sample can be soaked into fixation solution and then you can make a sample block (frozen / paraffin) like the way to make tissue block.